/   Home   /   Newsroom   /   News Updates

Chinese Scientists Create Pig Model of Huntington's Disease

Mar 30, 2018     Email"> PrintText Size

A Chinese team of scientists has established a pig model of Huntington's disease (HD), an inherited neurodegenerative disease, using genetic engineering technology.

In a study published in Cell on Thursday, researchers anticipated that the pigs could be a practical way to test treatments for HD, which is caused by a gene encoding a toxic protein that causes brain cells to die.

Although genetically modified mice have been used widely to model neurodegenerative diseases, they lack the typical neurodegeneration or overt neuronal loss seen in human brains, according to corresponding author Li Xiaojiang, professor of human genetics in Jinan University and who runs a lab at Emory University School of Medicine.

The pig HD model is an example that suggests large animal models could better model other neurodegenerative diseases, such as Alzheimer's and Parkinson's.

A HD pig could be an opportunity to test if the CRISPR-Cas9 gene editing technique can work in larger animals before clinical applications in humans.

Li said, in comparison with mice, treatments for affected nervous system tissues could be better tested in pigs, because their size is closer to that of humans. The pig model of HD also more closely matches the symptoms of the human disease.

Compared with non-human primate models, the pigs offer advantages of faster breeding and larger litter sizes, the researchers said.

Li collaborated with his colleagues at Jinan University and Chinese Academy of Sciences in Guangzhou. The pigs are now housed in Guangzhou.

"In pigs, the pattern of neurodegeneration is almost the same as in humans, and there have been several treatments tested in mouse models that didn't translate to human," said a co-senior author Li Shihua, professor of human genetics at Emory University School of Medicine.

Symptoms displayed by the genetically altered pigs include movement problems. They show respiratory difficulties, which resemble those experienced by humans with HD and are not seen in mouse models of HD.

In addition, the pigs show degeneration of the striatum, the region of the brain most affected by HD in humans, more than other regions of the brain.

Huntington's disease is caused by a gene encoding a toxic protein (mutant huntingtin or mHTT), and mHTT contains abnormally long repeats of a single amino acid, glutamine. Symptoms commonly appear in mid-life and include uncontrolled movements, mood swings and cognitive decline.

Researchers used the CRISPR/Cas9 gene editing technique to introduce a segment of a human gene causing Huntington's, with a very long glutamine repeat region, into pig fibroblast cells.

Then somatic cell nuclear transfer generated pig embryos carrying this genetic alteration. The alteration is referred to a "knock in" because the changed gene is in its natural context. (Xinhua)

Attachment:

(Editor: CHEN Na)

Contact

Phone:
E-mail:

Related Articles

gene editing;PEI Duanqing;human gene editing

Chinese Scientist Participates in Human Gene Editing Committee

Feb 17, 2017

A Chinese scientist has participated in making recommendations for human gene editing that cover basic laboratory research, and somatic and germline (heritable) genome editing, according to the Chinese Academy of Sciences (CAS). Pei Duanqing, a researc...

gene editing;International Summit on Human Gene Editing

World Scientists Agree to Take Cautious Step toward Human Gene Editing

Dec 04, 2015

A three-day International Summit on Human Gene Editing, held this week in Washington, D.C., wrapped up with a statement on human gene-editing, agreeing to proceed with gene editing research and application going forward, but with cautious steps.

gene editing;gene knockout;DNA;CRISPR-Cas9

First Gene-Edited Dogs Reported in China

Nov 02, 2015

Man’s best friend is now his newest genetic engineering project. Scientists in China say they are the first to use gene editing to produce customized dogs. They created a beagle with double the amount of muscle mass by deleting a gene called myostatin.

Contact Us

Copyright © 2002 - Chinese Academy of Sciences